- Postdoctoral Fellowship : Northwestern University (Neurobiology)
- Doctoral Degree: University of Wisconsin (Biochemistry)
- Bachelor's Degree: University of Michigan (Organic Chemistry)
- Office 513-558-6099
- Email kleenesj@ucmail.uc.edu
The Kleene lab studies signaling in primary cilia as it relates to kidney disease. Primary cilia are present on most mammalian cells. They influence fundamental cellular processes, and defects in primary cilia contribute to kidney disease, cancer, and obesity. Our work focuses on polycystic kidney disease (PKD), a renal disease that is often caused by defects in ion-conducting channels in the primary cilia. To that end, we have developed...
Peer Reviewed Publications
Kleene, Steven J 2022. Regenerative calcium currents in renal primary cilia Frontiers in Physiology, 13 , 894518
Kleene, Steven J 2022. Regenerative Calcium Currents in Renal Primary Cilia. Frontiers in physiology, 13 , 894518
Kleene, Steven J; Kleene, Nancy K 2021. Inward calcium current through the polycystin-2-dependent channels of renal primary cilia American Journal of Physiology Renal Physiology, 320 , F1165
Kleene, Steven J; Kleene, Nancy K 2021. Inward Ca2+ current through the polycystin-2-dependent channels of renal primary cilia. American journal of physiology. Renal physiology, 320 6, F1165-F1173
Kleene, Steven J; Siroky, Brian J; Landero-Figueroa, Julio A; Dixon, Bradley P; Pachciarz, Nolan W; Lu, Lu; Kleene, Nancy K 2019. The TRPP2-dependent channel of renal primary cilia also requires TRPM3. PloS one, 14 3, e0214053
Kleene, Steven J; Kleene, Nancy K 2017. The native TRPP2-dependent channel of murine renal primary cilia American Journal of Physiology Renal Physiology, 309 , F96
Siroky, Brian J; Kleene, Nancy K; Kleene, Steven J; Varnell, Charles D; Comer, Raven G; Liu, Jialiu; Lu, Lu; Pachciarz, Nolan W; Bissler, John J; Dixon, Bradley P 2017. Primary cilia regulate the osmotic stress response of renal epithelial cells through TRPM3 American Journal of Physiology Renal Physiology, 312 , F791
Nauli, Surya M; Pala, Rajasekharreddy; Kleene, Steven J 2016. Calcium channels in primary cilia Current Opinion in Nephrology and Hypertension, 25 , 452
Flannery, Richard J; Kleene, Nancy K; Kleene, Steven J 2015. A TRPM4-dependent current in murine renal primary cilia American Journal of Physiology Renal Physiology, 309 , F697
Kleene, Steven J; Van Houten, Judith L 2014. Electrical Signaling in Motile and Primary Cilia Bioscience, 64 , 1092-1102
Griff, Edwin R; Kleene, Nancy K; Kleene, Steven J 2012. A selective PMCA inhibitor does not prolong the electroolfactogram in mouse PLoS ONE, 7 , e37148
Kleene, Nancy K; Kleene, Steven J 2012. A method for measuring electrical signals in a primary cilium Cilia, 1 , 17
French, Donald A; Badamdorj, Dorjsuren; Kleene, Steven J 2010. Spatial distribution of calcium-gated chloride channels in olfactory cilia PLoS ONE, 5 , e15676
Kleene, Steven J 2009. Identifying olfaction's 'other channels' The Journal of Physiology, 587 , 4135-4136
Kleene, Steven J 2009. Limits of calcium clearance by plasma membrane calcium ATPase in olfactory cilia PLoS ONE, 4 , e5266
Badamdorj, Dorjsuren; Edwards, David A; French, Donald A; Kleene, Steven J 2008. Identification of Cl(Ca) channel distributions in olfactory cilia Mathematical Methods in the Applied Sciences, 31 , 1860-1873
Kleene, Steven J 2008. The electrochemical basis of odor transduction in vertebrate olfactory cilia Chemical Senses, 33 , 839-859
Smith, David W; Thach, Sokunthirith; Marshall, Erika L; Mendoza, Mary-Grace; Kleene, Steven J 2008. Mice lacking NKCC1 have normal olfactory sensitivity Physiology & Behavior, 93 , 44-49
Nickell, William T; Kleene, Nancy K; Kleene, Steven J 2007. Mechanisms of neuronal chloride accumulation in intact mouse olfactory epithelium The Journal of Physiology, 583 , 1005-1020
Flannery, Richard J; French, Donald A; Kleene, Steven J 2006. Clustering of cyclic-nucleotide-gated channels in olfactory cilia Biophysical Journal, 91 , 179-188
French, Donald A; Flannery, Richard J; Groetsch, Charles W; Krantz, Willam B; Kleene, Steven J 2006. Numerical approximation of solutions of a nonlinear inverse problem arising in olfaction experimentation Mathematical and Computer Modelling, 43 7-8, 945-956
Nickell, William T; Kleene, Nancy K; Gesteland, Robert C; Kleene, Steven J 2006. Neuronal chloride accumulation in olfactory epithelium of mice lacking NKCC1 Journal of Neurophysiology, 95 , 2003-2006
Pun, Raymund Y K; Kleene, Steven J 2004. An estimate of the resting membrane resistance of frog olfactory receptor neurones The Journal of Physiology, 559 , 535-542
Pun, Raymund Y K; Kleene, Steven J 2003. Contribution of cyclic-nucleotide-gated channels to the resting conductance of olfactory receptor neurons Biophysical Journal, 84 , 3425-3435
Nickell, W T; Pun, R Y K; Bargmann, C I; Kleene, S J 2002. Single ionic channels of two Caenorhabditis elegans chemosensory neurons in native membrane The Journal of Membrane Biology, 189 , 55-66
Pun, Raymund Y K; Kleene, Steven J 2002. Outward currents in olfactory receptor neurons activated by odorants and by elevation of cyclic AMP Cell Biochemistry and Biophysics, 37 , 15-26
Frings, S; Reuter, D; Kleene, S J 2000. Neuronal Ca2+ -activated Cl- channels--homing in on an elusive channel species Progress in Neurobiology, 60 , 247-289
Kleene, S J 2000. Spontaneous gating of olfactory cyclic-nucleotide-gated channels The Journal of Membrane Biology, 178 , 49-54
Kleene, S J 1999. Both external and internal calcium reduce the sensitivity of the olfactory cyclic-nucleotide-gated channel to cAMP Journal of Neurophysiology, 81 , 2675-2682
Kleene, S J 1997. High-gain, low-noise amplification in olfactory transduction Biophysical Journal, 73 , 1110-1117
Larsson, H P; Kleene, S J; Lecar, H 1997. Noise analysis of ion channels in non-space-clamped cables: estimates of channel parameters in olfactory cilia Biophysical Journal, 72 , 1193-1203
Kleene, S J; Pun, R Y 1996. Persistence of the olfactory receptor current in a wide variety of extracellular environments Journal of Neurophysiology, 75 , 1386-1391
Kleene, S J 1995. Block by external calcium and magnesium of the cyclic-nucleotide-activated current in olfactory cilia Neuroscience, 66 , 1001-1008
Kleene, S J 1994. Inhibition of olfactory cyclic nucleotide-activated current by calmodulin antagonists British Journal of Pharmacology, 111 , 469-472
Kleene, S J; Cejtin, H C 1994. Solving buffering problems with Mathematica software Analytical Biochemistry, 222 , 310-314
Kleene, S J; Gesteland, R C; Bryant, S H 1994. An electrophysiological survey of frog olfactory cilia The Journal of Experimental Biology, 195 , 307-328
Pun, R Y; Kleene, S J; Gesteland, R C 1994. Guanine nucleotides modulate steady-state inactivation of voltage-gated sodium channels in frog olfactory receptor neurons The Journal of Membrane Biology, 142 , 103-111
Kleene, S J 1993. Origin of the chloride current in olfactory transduction Neuron, 11 , 123-132
Kleene, S J 1993. The cyclic nucleotide-activated conductance in olfactory cilia: effects of cytoplasmic Mg2+ and Ca2+ The Journal of Membrane Biology, 131 , 237-243
Kleene, S J 1993. A simple intrapipette salt bridge Journal of Neuroscience Methods, 46 , 11-16
Kleene, S J 1992. Basal conductance of frog olfactory cilia Pflügers Archiv : European Journal of Physiology, 421 , 374-380
Kleene, S J; Gesteland, R C 1991. Calcium-activated chloride conductance in frog olfactory cilia The Journal of Neuroscience, 11 , 3624-3629
Kleene, S J; Gesteland, R C 1991. Transmembrane currents in frog olfactory cilia The Journal of Membrane Biology, 120 , 75-81
Lidow, M S; Gesteland, R C; Shipley, M T; Kleene, S J 1987. Comparative study of immature and mature olfactory receptor cells in adult frogs Developmental Brain Research, 31 , 243-258
Kleene, S J 1986. Bacterial chemotaxis and vertebrate olfaction Experientia, 42 , 241-250
Lidow, M S; Kleene, S J; Gesteland, R C 1986. Suppression of mitotic activity and synchronization of cell development in olfactory epithelium Developmental Brain Research, 28 , 145-162
Kleene, S J; Gesteland, R C 1983. Dissociation of frog olfactory epithelium Journal of Neuroscience Methods, 9 , 173-183
Kleene, S J; Gesteland, R C 1981. Dissociation of frog olfactory epithelium with N-ethylmaleimide Brain Research, 229 , 536-540
Kleene, S J; Hobson, A C; Adler, J 1979. Attractants and repellents influence methylation and demethylation of methyl-accepting chemotaxis proteins in an extract of Escherichia coli Proceedings of the National Academy of Sciences of the United States of America, 76 , 6309-6313
Kleene, S J; Toews, M L; Adler, J 1977. Isolation of glutamic acid methyl ester from an Escherichia coli membrane protein involved in chemotaxis The Journal of Biological Chemistry, 252 , 3214-3218